CETRI

Stahl Lab

The Stahl lab investigates the contribution of the innate immune system to tissue injury and inflammation following ischemia and reperfusion injury. During the past several years, Dr. Stahl's lab has focused on the contributions of the complement pathways responsible for the initiation of complement activation following ischemia/reperfusion, as well as the role of the early versus late complement components in mediating inflammation and tissue injury.

The main findings from the Stahl lab have demonstrated the significant importance of the terminal/late complement components (e.g., C5a and C5b-9) in mediating tissue inflammation and injury compared to the early complement components (e.g., C3a, C3b, etc.) in ischemia/reperfusion and sepsis. Additionally, the seminal work from the Stahl lab has established the importance of mannose-binding lectin (MBL) initiating complement activation, tissue injury and inflammation following ischemia/reperfusion. Current studies and recent publications by Dr, Stahl’s lab have investigated the importance of MBL in coagulation abnormalities, as well as acute hyperglycemia and its role in the cardiomyopathies associated with diabetes mellitus.

Model of the recognition molecules MBL and ficolins in association with the MBL/ficolin associated serine proteases
A model of the recognition molecules MBL and the ficolins in association with the MBL/ficolin associated serine proteases (MASPs) are shown. The possible roles of the MASPs in activation of the complement system and other cascade systems and subsequent MBL/fiscolin associated protein-1 (MAP-1) inhibition are indicated. Hmw = high molecular weight.

Principal Investigator

Gregory L. Stahl, PH.D.

Gregory L Stahl

Gregory L. Stahl, Ph.D. is the Paul Allen Distinguished Professor at Brigham and Women's Hospital, Harvard Medical School. He obtained his PhD in Cardiovascular Physiology from Thomas Jefferson University in 1988. Dr Stahl is a full Professor of Anaesthesia at Harvard Medical School.

  • BWH Distinguished Chair in Anesthesiology.
  • Full Professor of Anaesthesiology Harvard Medical School.

Current Personnel 2014–2015

  • Margaret MorrisseyMargaret Morrissey, C.V.T Laboratory Investigator
  • Vasile I. PavlovVasile I. Pavlov, M.D. Research Fellow
  • Masayuki Ozaki, M.D., Ph.D.Masayuki Ozaki, M.D., Ph.D. Research Fellow

Select Recent Publications

  • Takahashi K, Chang W-C, Takahashi M, Pavlov V, Ishida Y, La Bonte L, Shi L, Fujita T, Stahl GL*, Van Cott EM* (*co-senior authors). “Mannose-binding lectin and its associated proteases (MASPs) mediate coagulation and its deficiency is a risk factor in developing complications from infection, including disseminated intravascular coagulation.” Immunobiology 2011; 216:96-102 PMCID: PMC2912947 (cover issue)
  • Zou, C, La Bonte LR, Pavlov VI, Stahl GL. “Murine hyperglycemic vasculopathy and cardiomyopathy: whole-genome gene expression analysis predicts cellular targets and regulatory networks influenced by mannose binding lectin.” Frontiers in Immunology 2012; 3:15. doi: 10.3389/fimmu.2012.00015 PMC3286603
  • Van de Pol P, Schlagwein N, van Gijlswijk DJ, Berger SP, Roos A, Bajema IM, de Boer HC, de Fijter JW, Stahl GL, Daha MR, van Kooten C. “A crucial role for mannan-binding lectin in renal ischemia/reperfusion injury.” American Journal of Transplantation 2012; 12:877-87
  • La Bonte LR, Pavlov VI, Tan YS, Takahashi K, Takahashi M, Banda NK, Fujita T, Stahl GL. “MBL-associated serine protease -1 (MASP-1) is a significant contributor to coagulation in a murine model of occlusive thrombosis.” Journal of Immunology 2012; 188:885-91 PMC3253146
  • Pavlov VI, La Bonte LR, Baldwin WM, Markiewski M, Lambris J, Stahl GL. “Absence of mannose-binding lectin prevents hyperglycemic cardiovascular complications.” American Journal of Pathology 2012; 180:104-112 PMC3338344
  • Pavlov VI, Skjoedt M-O, Tan YS, Rosbjerg A, Garred P, Stahl GL. “Endogenous and natural complement inhibitor attenuates myocardial injury and arterial thrombogenesis.” Circulation 2012; 126:2227-
  • Orsini F, Villa P, Parrella S, Zangari R, Zanier ER, Gesuete R, Stravalaci M, Fumagalli S, Ottria R, Reina JJ, Paladini A, Micotti E, Ribeiro-Viana R, Rojo J, Pavlov VI, Stahl GL, Bernardi A, Gobbi M, De Simoni M-G. “Targeting mannose binding lectin confers long lasting protection with a surprisingly wide therapeutic window in cerebral ischemia.” Circulation 2012; 126:1484-94
  • Stahl GL, Shernan SK, Smith PK, Levy JH. “Complement activation and cardiac surgery: A novel target for improving outcomes.” Anesthesia and Analgesia (Invited Review) 2012; 115:759-71
  • Gorsuch WB, Chrysanthou E, Schwaeble WJ, Stahl GL. “The complement system in ischemia-reperfusion injuries.” Immunobiology (Invited Review) 2012; 217:1026-33
  • Full publication listing